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Feeder Cells
(Primary Mouse embryonic Fibroblasts, PMEFs, used for generating knock out mouse production and as feeder for human stem cells)
Primary Mouse Embryonic Fibroblasts (PMEFs) are isolated from mouse embryos at E13.5 day. These are primary cells and as such have a limited life span in culture. A healthy state of culture is maintained during expansion. We use virus and pathogen free mice.
Monolayers of mitotically inactivated feeder cells are essential for successful cultivation of mouse embryonic stem (ES) cells. ES cells are used in gene targeting and knock out mouse production. When grown under optimal conditions along with feeder cells, ES cells can be maintained in culture for many passages, retaining their non-differentiated morphology and pluripotent properties. The feeder cells secrete several important growth factors into the medium that may play roles in maintaining the pluripotency of ES cells
(A) Primary Mouse Embryonic Fibroblasts (PMEFs), passage 1
These cells are produced in facility licensed by the USDA, fully accredited by AAALAC. MEFs are prepared from embryos at E 13.5. These cells are from passage 1 and can be passaged to 6-8 times. These cells are used as STOCK CULTURES.
Catalog numbers:
PMEF 01-01/NEO
neomycin resistant, 3.2 million cells/vial; set of 2 vials.
PMEF 01-01/HYGRO
hygromycin resistant, 3.2 million cells/vial; set of 2 vials
(B) Primary Mouse Embryonic Fibroblasts (MEFs, Feeder cells), Ready to use, mitotically arrested
We are also producing ‘Ready to Use ‘mouse embryonic fibroblasts. These cells are from passage 3 and mitotically arrested by mitomycin C. These cells can be used directly as a feeder cells.
Catalog number
PMEF 01-01/NEO/M
neomycin resistant, 5 million cells/vial; set of 5 vials
PMEF 01-01/HYGRO/M
hygromycin resistant, 5 million cells/vial; set of 5 vials
PMEF 01-01/PURO/M
puromycin resistant, 5 million cells/vial; set of 5vials
We produce these cells in large quantities and can supply at a very affordable price. For more information, please contact us.
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